Paraguay have set the stage for a high-stakes showdown with the Socceroos, defeating Turkey 1-0 despite Miguel Almiron becoming the first player to be sent off at the World Cup for covering his mouth.
Matias Galarza's goal, scored a tick over one minute into the clash between two of Australia's Group D rivals, proved the difference in Santa Clara.
The result means victory in the match between the Socceroos and Paraguay, kicking off at 1200 AEST on Friday, will be rewarded with second spot in the group.
A draw between Australia and Paraguay would leave both sides level on four points, but Tony Popovic's side would finish second because of their superior goal difference.
The group's third-placed team will still have a chance to reach the knockout phase, while Turkey are guaranteed to finish fourth and head home after their dead rubber against the table-topping United States.