Twitter's head of safety and content moderation, Ella Irwin, says she has resigned, becoming the second executive to quit the role since Elon Musk bought the social media company in October.
A former executive at Amazon and Google, Ms Irwin joined Twitter last June, shortly before the company's former safety chief Yoel Roth resigned in November.
Ms Irwin's departure comes as the platform has struggled to retain advertisers, with brands wary of appearing next to unsuitable content.
Tesla chief and SpaceX founder Mr Musk announced earlier this month that he hired Linda Yaccarino — former NBCUniversal advertising head — to become Twitter's new CEO.
He said he would be transitioning into the role of executive chair and chief technology officer, overseeing product, software and system operations.
Fortune earlier reported that Ms Irwin's internal Slack account appeared to have been deactivated.
Ms Irwin declined to further comment on her resignation and Mr Musk did not immediately respond to a media request.
An email to Twitter returned an automated reply of a poop emoji.
Since Mr Musk's acquisition, Twitter has cut costs dramatically and laid off thousands of employees, including many who had worked on efforts to prevent harmful and illegal content, protect election integrity and surface accurate information on the site.
Mr Musk has recently promoted a feature called Community Notes, which lets users add context to tweets, as a way to combat misleading information on Twitter.
The company is facing increasing scrutiny from regulators over its moderation efforts.
Twitter withdrew from a voluntary agreement with the European Union to tackle disinformation, while saying it was committed to complying with upcoming internet rules in the EU.
Last week, EU industry chief Thierry Breton warned Twitter that it would not be able to avoid its legal obligations in Europe.
Reuters/ABC