Kate Forbes has insisted trans women are "biological males" as further questions are asked about her socially conservative views.
The finance secretary's views on the gender reform debate put her at odds with many senior SNP parliamentarians as well as the Scottish Greens, who signed a power-sharing deal with the party.
Asked if she believed a trans woman is a woman, Forbes told ITV Border today: "I believe that a trans woman is a biological male, who identifies as a woman."