Matildas captain Sam Kerr has been ruled out of the two matches against Olympic champions Canada due to a foot injury.
The star striker will remain in London for treatment with her place in the squad taken by midfielder Sarah Hunter, the 20-year-old earning her first senior national team call-up.
The news comes a day after it was confirmed Charlotte Grant (hamstring), Holly McNamara (knee), Courtnee Vine (hamstring) and World Cup hero goalkeeper Mackenzie Arnold would also miss the Canada games.