Stirling Council has rejected Wolfcraig Distillers' plans for a £20m whisky distillery and visitor centre on the outskirts of Stirling.
The Local Review Body turned down the application after the plans were initially blocked due to concerns around the potential impact on businesses in the city centre.
The proposals could have created more than 50 jobs at a new site at Craigforth, according to Wolfcraig, which is now looking at alternative locations.