Tokyo: Antisense oligonucleotides (ASOs), specialised molecules that may regulate RNA and affect protein creation, are being injected directly into the cerebrospinal fluid, the region around the brain and spinal cord, as a prospective treatment for a variety of brain illnesses.
However, when ASOs are given in this manner, they frequently result in significant adverse effects. According to a new study published in Molecular Therapy--Nucleic Acids, such negative effects are induced by calcium imbalances in the brain and can be alleviated by calcium-balance modulators.
Many brain diseases are thought to be caused by specific proteins. ASOs can be created to bind to the RNA that provides a template for a disease-related protein, usually with the aim of making more or less of the protein. To alter protein production in the brain only, ASOs are then injected directly into patients' cerebrospinal fluid, which flows in and around the whole brain and spinal cord.
However, only one such ASO treatment is currently available, to treat spinal muscular atrophy. Many other promising ASOs can induce neurotoxicity (that is, they cause disturbances of consciousness or motor function), which is experienced as unpleasant and sometimes life-threatening side effects. Because the reason for this neurotoxicity is relatively unknown, treating ASO-related neurotoxicity or creating new ASOs with low neurotoxicity is difficult. The researchers from Tokyo Medical and Dental University (TMDU) wanted to address this problem.
"We used three different ASOs that we know are neurotoxic and injected them into the cerebrospinal fluid of mice," says lead author Chunyan Jia. "The mice showed many abnormal behaviors that indicated acute neurotoxicity, and these behaviors were correlated with changes in calcium levels, as measured in other experiments with neuronal cells."
Specifically, when the neurotoxic ASOs were used to treat cells, they reduced the levels of free calcium within the cells. Importantly, these reductions were associated with neurotoxicity levels in the mice. The results indicated that calcium levels within cells are important for modulating ASO neurotoxicity, and suggested ways of modifying the calcium balance to reduce neurotoxicity.
"Our findings have important implications for developing effective ASO therapies with fewer harmful side effects," explained Kotaro Yoshioka, senior author.
"As well as suggesting drugs that may be used alongside ASOs to reduce neurotoxicity, we also reported a relationship between certain nucleotide sequences in ASOs and greater neurotoxicity; this information may be useful when choosing potential ASOs for clinical use." said Takanori Yokota, director of the research group. (ANI)
