Clubs will be allowed to name up to seven substitutes and make five substitutions in EFL Championship, League One and League Two matches from the 2022-23 season.
Teams were previously permitted to field three substitutes from seven nominated.
The EFL announced the increase following its annual general meeting on Friday, when clubs approved a number of regulation changes which will come into effect immediately.
As part of the new substitution rules, each club is only permitted a maximum of three opportunities to make changes during the fixture, plus an opportunity at half-time.
Managers may make more than the one substitution on each occasion.
