A Cabinet minister has said the chant "from the river to the sea, Palestine will be free" is "extremely offensive" and anti-Semitic.
Culture Secretary Lucy Frazer spoke on Wednesday about the chant, which has been used at pro-Palestinian demonstrations in London and around the UK since the Hamas attack on October 7 and Israel's subsequent bombing of Gaza.
She told LBC Radio: "That is a chant that is calling for the eradication of the state of Israel and by analogy, therefore, the eradication of the Jews within Israel.
"I think that is extremely offensive. I think it is anti-Semitic."
Ms Frazer, who has a Jewish grandmother, also said: "I feel for the Jewish community, who are feeling very unsafe.
"Whether you’re in a senior position, or you’re a young kid on the street who wants to wear a Star of David around your neck, or you’re a primary school child who can’t wear their blazer to school, I think that is a very, very sad state of affairs in our country."
The Metropolitan Police should also keep the "very provocative" pro-Palestinian march planned for Armistice Day in London "under review", Ms Frazer said.
She told LBC: “I think that the police need to, and have said that they will, continue to look at intelligence and will impose conditions.
"And they’ve already set out where events will take place, but I think they should keep it under review.
"I think this is a very provocative event. What we’re talking about this weekend is respecting those who have fought for our country, we are respecting the fallen."
She said she is "concerned about activities that are taking place on these marches" and that there have been arrests involving "incitement to racial hatred".
Ms Frazer, who is Jewish, said she has not been subjected to abuse since the crisis in the Middle East escalated in recent weeks.
Her comments come as Prime Minister Rishi Sunak vowed to hold the Met chief "accountable" for allowing the pro-Palestinian march to take place on Armistice Day.
Sir Mark Rowley on Tuesday said the legal threshold for banning it had not been met so far and it would be allowed to go ahead unless the intelligence picture changed.
Mr Sunak said he would be meeting Sir Mark later on Wednesday to discuss the situation.
He warned: "This is a decision that the Metropolitan Police Commissioner has made. He has said that he can ensure that we safeguard remembrance for the country this weekend as well as keep the public safe. Now, my job is to hold him accountable for that."
He added: "We’ve asked the police for information on how they will ensure that this happens. I’ll be meeting the Metropolitan Police Commissioner later today to discuss this. My view is that these marches are disrespectful."
Chris Nineham, from the Stop the War coalition, one of the groups organising Saturday’s march from Hyde Park, said he and his colleagues would do "everything we can... to make sure that there is nothing anti-Semitic, nothing calling for violence" during the protest.
He also insisted that the march "isn’t about religion, it isn’t about race" but instead was intended to protest "about a humanitarian catastrophe" in Gaza.
More than 10,500 Palestinians have been killed in Gaza — two-thirds of them women and minors, according to the Health Ministry in the Hamas-run territory.
More than 2,300 are believed to have been buried by strikes that in some cases have demolished entire city blocks.
More than 1,400 people have died in Israel since the start of the war, most of them civilians killed by Hamas militants during the October 7 incursion.
Scores of hostages were also taken that day. Israel says 32 of its soldiers have been killed in Gaza since the ground offensive began, and Palestinian militants have continued to fire rockets into Israel on a daily basis.
