Che Adams has been ruled out of Scotland's European qualifier against Spain on Tuesday night.
The Southampton striker limped off against Cyprus on Sunday following a calf strain and he has now exited Steve Clarke's squad.
Hearts striker Lawrence Shankland has been called up and he is available for selection for the Hampden showdown in midweek.
There are also injury fears for Celtic defender Anthon Ralston, who has also left the squad, despite being a late call-up.
The extent of his knock remains to be seen, but his place in the squad has been left vacant with no replacement called up.
Steve Clarke wants his Scotland players to build on their encouraging 3-0 win over Cyprus when Spain visit Hampden Park on Tuesday night.
Midfielder John McGinn scored his 16th international goal after 21 minutes of their European Championship qualifying Group A opener at the national stadium, but it was not until second-half substitute Scott McTominay grabbed a late double that the Tartan Army could relax.
Unfancied Cyprus finished the game with 10 men after Nicholas Ioannou was sent off for booting the ball away in frustration to pick up his second yellow card.
It is the first time since 2006 that Scotland have won their opening Euros qualifier and the boss wants to take the positives into what is sure to be a much tougher game against Spain.
“It’s a better start than we’ve had to a European Championship campaign in a long time,” said Clarke, who revealed Che Adams went off with a tight calf and that him recovering in time for Spain would be “optimistic”.
“Against the teams seeded lower than you, it’s important to pick up maximum points at home,” added the manager.
“Cyprus were good and organised but we managed to contain them. There wasn’t much threat on our goal.
“But at 1-0 it’s always nervous. It’s a really good start but we have to try and back it up on Tuesday against the Spanish.
“When you are playing one of the Pot One teams, you have to play as well as we can play. That is what we are aiming to do.
“We will prepare the lads well, we will get them recovered and we will be ready to go again but we have to play as well as we can play.
“We always want to improve. You always want the perfect performance and it’s rare that you get it.
“But you have to look for it all the time. In saying that, if we don’t get it on Tuesday night and still get a result, we’ll come away happy.”
