Boris Johnson was subjected to a second savage dig tonight - as he watched Platinum Party at the Palace from the royal box.
The Prime Minister was in attendance at the event at Buckingham Palace, which saw some of the biggest names in music and showbiz come out to celebrate Queen Elizabeth's incredible 70 year reign on the throne.
Boris and Partygate were mentioned earlier on in the night by comedian Lee Mack, and when Stephen Fry took to the stage just over an hour or so later, he couldn't help but make a dig at the Prime Minister's expense too.
Introducing Prince Charles' sweet speech to his mother, Stephen said: "As a nation, we have been lucky, lucky, lucky above all others to have had our Queen to represent us over 70 years of Trooping the Colour, royal tours, Christmas Day at three in the afternoon.
"How many local sewage works has our Majesty opened with a bright smile? How many plaques unveiled? How many trees planted, ribbons cut, ships launched?" he added as he noted all the great work Her Majesty had done.
But it was his final comment that left the audience gasping, as he took aim at the Prime Minister.
"How many prime ministers tolerated – for that alone, no admiration is high enough," he concluded, leaving people both at home and those in attendance at the event itself in shock.
Fans tuning in from home found the whole thing hilarious and took to Twitter to share their thoughts, with one writing: "Drone Corgis and @stephenfry taking a swipe at @BorisJohnson, what a fantastic evening. #PlatinumPartyatthePalace."
Another added: "Safe to say @BorisJohnson was the laughingstock this evening #PlatinumPartyatthePalace."
Earlier in the evening, Lee Mack made a joke at the expense of the Prime Minister, as he introduced the show following Queen and Adam Lambert's opening performance.
As he spoke of how amazing it was for the gig to be taking place just steps away from the Buckingham Palace gates, he added: "Finally we can say the words Party and Gate and it be a positive," earning a raucous sound of laughter from the audience.