Unions and environmentalists will join traditional owners on a two day convoy from Narrabri to Newcastle to protest against the Santos' Narrabri Gas Project and associated Hunter Gas Pipeline.
The "Picket the Pipeline" convoy will leave Narrabri on Saturday and arrive in Civic Park, Newcastle on Sunday afternoon.
It will stop along the 400 kilometre trek route to meet with impacted communities and landholders.
The convoy comes as Gomeroi Traditional Owners return to the Native Title Tribunal, after an appeal to the Federal Court found an earlier determination by the tribunal should have considered climate change when considering whether the project was in the public interest.
"We are standing with the traditional owners and the concerned landholders along the route to oppose this (project) and call on the Minn's government to prevent it from going ahead," Lock the Gate's Hunter coordinator Steve Phillips said.
He said landowners in Newcastle and the Lower Hunter were equally concerned as those on the Northern Tablelands.
"There are a lot of potential direct impacts in the Hunter when you consider the corridor is 200 metres wide. This is a serious development that would rip through some important agricultural land and biodiversity, including koala habitat."
Landowners living on the 413 kilometre Hunter Gas Pipeline route hit out at an advertising campaign earlier this year that claimed normal agricultural activity will be permitted above the pipeline.
They included 120 Hunter Gas Landholder Rights Alliance members who live on 100 properties between Hebden near Muswellbrook and Millers Forest.
All of the alliance members, who represent almost 60 per cent of the properties in the area, have indicated they do not want the pipeline on their land.
"Landholders and the communities that support them have shown powerful opposition to the Hunter Gas Pipeline, yet Santos and the Minns Government still don't seem to get it," Lock the Gate Alliance NSW Coordinator Nic Clyde said.
"As well as threats to culture, farming and water resources, analysis has also revealed more than 1000 hectares of Koala habitat could be impacted by the Hunter Gas Pipeline.
"We're calling for proactive action by the Minns Government to rule out new gasfields in NSW and ensure these pipelines that would carve up farmland and natural areas are not approved."
Karra Kinchela said traditional owners along the pipeline route were united in their opposition to both the Narrabri and Hunter Gas Pipeline projects.
"We don't want hundreds of coal seam gas wells drilled into the sacred Pilliga Forest, clearing the trees, and poisoning our water," she said.
"Santos has ignored our opposition to this project and tried to force it on us, but there's no way we're going to let this company lay waste to the Pilliga and our communities."
