The chairman of the Young Conservatives network has temporarily stepped down from his role after calling Birmingham "a dump".
Daniel Grainger tweeted the comment on Saturday, prompting the West Midlands Mayor to urge him to leave the city.
The Young Conservative Network told members the decision was made following talks with CCHQ.
Mr Grainger deleted the tweet after it went viral as Conservative members were arriving in the city on the eve of the party's conference.
He apologised and said his tweet “was not about the city” and that he had been the victim of a mugging attempt earlier on Saturday.
But Mayor Andy Street told him to leave and "take anyone with the same views with you".
In an email to young Tory members, the network said: "Over the past three years, we have strived, as an organisation, to facilitate a way for young members to be able to connect with fellow members, but also people in Government, past and present.
"The events of the past day do not infer any opinions or stances of our organisation.
"All our opinions are our own, and do not reflect our work."
The Company Secretary Thomas Moss will act as chair of the network for the time being.
But it was not the first time he had made critical comments about Birmingham on Twitter.
In April he tweeted, "Birmingham is firmly the worst city in the UK".
In a string of tweets apologising, Mr Grainger wrote: “My tweet in relation to Birmingham was not about the city or its people.
"I’ve always enjoyed my visits to your city, but this morning I was greeted by an individual who threatened me with a mugging.
“I was angry and tweeted without thinking, I apologise for any offence caused.
“I’d also like to directly apologise to (Andy Street), the (Conservatives) and other representatives of Birmingham for my ill conceived comments. I have now deleted the tweet.”
Mr Street and Labour MP Jess Phillips were among those to pour scorn on the comment.
“Off you pop then, and take anyone with the same views with you. Try and take some time to grow up and understand just why this city is so special whilst you’re gone,” the mayor wrote.
Ms Phillips tweeted: “I see the Tories doing their best to endear themselves.”
Mr Street added: “I may be a Conservative, but I’m also a bloody proud Brummie.
"I won’t have anyone who’s never lived or breathed this place trying to put us down.”
The exchange comes against a backdrop of wider divisions emerging within the Tory party, after the Chancellor’s mini-budget prompted market turmoil.
