Maddison Inglis's inspired run at the Australian Open has come to an end, with the local wildcard going down in three sets to Estonia's Kaia Kanepi.
Inglis — ranked 133 in the world — had never won a singles match at a major until this week and she appeared on the verge of causing an upset against Kanepi when she won the first set.
But Kanepi's experience proved a factor, with the 36-year-old winning 10 of the final 11 games to triumph 2-6, 6-2, 6-0 in an hour and 31 minutes on a sun-bathed Rod Laver Arena.
Inglis's loss leaves world number one Ash Barty as the only Australian left in the women's main draw.
The 24-year-old wildcard from Perth provided a compelling storyline in the first week of the Open, having stunned US Open finalist Leylah Fernandez with a straight-sets win in the first round.
She defeated American qualifier Hailey Baptiste to reach the third round, but beating Kanepi — who has been ranked as high as 15 in the world — proved to be a challenge too far.
Inglis will enjoy a significant rankings boost following her showing at Melbourne Park, while she will be $221,000 richer as reward for making the last 32.
She had only earned $530,715 in career prize money prior to the tournament.
"I think it's been an amazing week for me," Inglis said.
"I just feel like I've taken a bit of belief out of it, that I can play at this level and I belong here.
"I think I've just got to be able to maintain it longer against those really good players for three, or four or five matches in a row.
In the first set, Inglis showed no signs of being overawed facing Kanepi on Melbourne Park's centre court, with the temperature hovering above 30 degrees Celsius.
She burst out of the blocks in the opening set, racing out to a 4-0 lead.
The West Australian broke Kanepi in her opening two service games, with the Estonian finding her powerful groundstrokes nullified by her lower-ranked opponent.
Kanepi got on the scoreboard when she held serve in the fifth and seventh games, but the damage had been done.
Inglis confidently won her next two service games to claim the first set in 31 minutes.
As Inglis walked to her chair at the end of the set, she noticeably took a deep breath as if the enormity of what she was trying to achieve may have started hitting home.
Perhaps it did, as she lost her momentum in the second set, failing to win any of her four service games.
She did break twice, but Kanepi levelled the match to force a deciding set.
Inglis again struggled with her serve in the third set, as Kanepi broke three times while denying the Australian even a single game.
Kanepi has now reached the last 16 in Melbourne for the fourth time in her career.
She will play Belarusian second seed Aryna Sabalenka, who beat 31st-seeded Markéta Vondroušová of the Czech Republic 4-6, 6-3, 6-1.
In other results, Romanian two-time major winner and former world number one Simona Halep wasted little time in ending the tournament of Montenegro's Danka Kovinić with a 6-2, 6-1 victory.
Belgian 19th seed Elise Mertens made light work of China's Shuai Zhang, winning 6-2, 6-2.
American Danielle Collins and France's Alizé Cornet had more difficulty in booking their fourth-round berths, with both winning matches lasting well over two hours.
Collins — the 27th seed and 2019 semi-finalist — lost the first set against Denmark's Clara Tauson, before bouncing back to win 4-6, 6-4, 7-5.
Cornet made it through to the Australian Open fourth round for the first time since 2009 after battling past Slovenian 29th seed Tamara Zidanšek 4-6, 6-4, 6-2.