Jacinta Allan will face a vote of no confidence as the Victorian premier's would-be replacement seeks to hold her feet to the fire.
Opposition Leader Jess Wilson will give notice to state parliament's lower house on Thursday that the coalition plans to move a motion of no confidence in the premier and her ministers.
Only one motion of no confidence can be moved each four-year term and can lead to the dissolution of parliament if passed, well before the scheduled state election on November 28.
Ms Wilson said there must be accountability for government failings, citing record crime, skyrocketing net debt, major project cost blowouts and delays, and the 2026 Commonwealth Games fiasco.
"Victoria needs a fresh start and that begins with changing the government and changing the premier," the state Liberal leader said.