Presenter Adam Hills apologised after The Last Leg received criticism over their World Cup parody song.
The song, which followed the tune of Three Lions, started with a repetition of "It's (be)coming home-ophobic."
Adam, who hosts the sports comedy show, sang their version of the 'anthem' after England beat Iran 6-2 earlier this week.
The song references many of the controversies relating to the World Cup's host country, Qatar, including the mistreatment of migrant workers and the illegality of homosexuality.
One specific lyric shocked viewers, which had them take to social media to slam Adam and the show for being 'gross'.
Referencing the violence LGBTQ+ people face, they sang: "But in Qatar if Alan Carr toured/ And was joined by Boy George.
"And then even RuPaul/ They could end up/ Three heads on a spike.”
Taking to Twitter, a furious fan wrote: "Using the imagery of dead queer people when we are murdered every day and the racist notion that Qatari people will put heads on spikes is gross."
One user re-posted the clip on their Instagram page along with the caption: "@adamhillscomedy used to like you. People were just shot and killed in Colorado for being LGBTQIA. For being themselves. Be Ashamed."
Adam took notice of the backlash and responded to the user: "I’m sorry. This song was intended to be a protest at the World Cup being held in Qatar where it is illegal to be gay.
He added that the segment was shot and filmed before the horrific shooting.
The episode with the parody originally aired on November 18.
However, The Last Leg's official page shared the clip of the song on November 21, days after five people were killed in Colorado.
A mass shooting took place on November 19 at the LGBTQ+ venue, Club Q, in Colorado Springs.
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Five people died and 18 people were injured.
The backlash over the song comes at the same time FIFA has been criticised for choosing Qatar as the World Cup's host country.
Qatar is one of the most dangerous countries in the world for members of the LGBTQ+ community.
Same-sex relationships are outlawed in the country, with homosexuality even punishable by death under Qatari law.
David Beckham is also under fire for agreeing to a £10million deal to be an ambassador for the World Cup.
