Parents have been left angered after a school banned its pupils from wearing skirts and introduced a trousers-only uniform policy.
Haywood Academy in Burslem, Stoke-on-Trent, brought in the rule for the start of the academic year.
An online petition against the move has already gathered more than 110 signatures and was set-up by furious mum-of-four Claire Rigby.
Claire, 41, claims two of her daughters have been threatened with "seclusion" if they turn up to lessons in skirts, StokeonTrentLive reports.
She says many students are choosing to defy the ban due to "double standards" as staff and teachers can still wear what they want.
Claire adds that the school carried out a survey before the summer break and chose to opt for tailored black trousers for "affordability reasons".
Other schools across the UK have barred children from wearing skirts under new gender neutral rules but it is not yet clear whether this is the case with Hayward Academy.
Other issues around wearing skirts found across different parts of the country include skirts being rolled up to make them too short.
Claire's older daughter, Louise Coates, 15, is a prefect, while younger daughter Charlie Coates, 12, is going into year eight.
Both have only ever worn skirts and plan to continue to do so despite the risk of being put in isolation.
"The only option is black tailored trousers and no skirts. It hasn't gone down well with my two daughters," said Claire.
"There is a group of Year 10 girls that gathered lots of evidence from pupils, ex-pupils and anonymous staff members to show that it's wrong and they don't agree with taking skirts away.
"They had a meeting with the headteacher which was right before the break up at the end of term. Over the summer there have been numerous parents that have an email regarding it and nobody has had anything back."
She added: "My girls won't wear trousers. My daughter is the biggest tomboy in the world, she plays football and she still doesn't want to wear trousers.
"There are a few pupils who have backed down because they don't want to be put in seclusion. But my daughters don't agree with it.
They've had the decision taken away. They want to stand up for what they believe in.
"The school aren't telling female teachers that they have to wear trousers. It is just wrong."
Haywood Academy has been approached for comment by The Mirror.