The Queen's vigil showed that Prince Harry has lost the trust of his family, according to a royal expert.
The Duke of Sussex was denied the right to wear the monarch's "ER" cypher on his military uniform as he held vigil over her coffin in Westminster Hall.
Only royals who are in active service to the monarch are allowed to wear the prestigious letters on their shoulder.
Harry was not eligible for the honour after he and his wife stepped down from royal duties following Megxit.
However, Prince William was allowed to wear the cypher at the Queen's vigil.
Royal journalist Robert Jobson, writing in The Sun, said: "He will have complained the Queen’s cypher was removed from his uniform as he held vigil over her coffin.
"But this insignia is only ever awarded to those in active service to the monarch, something Harry left in 2020.
"It is moments like these which show the family’s trust in Harry has been broken."
It comes after a body language said Prince William and Harry stood out at as the brothers were united in grief at the Queen's vigil.
William and Harry, dressed in military uniforms, joined their cousins for a 15 minute moment of reflection at Westminster Hall yesterday evening.
As Her Majesty's grandchildren stood silently to pay their respects, it was the famous duo who caught the eye as they walked apart from one another, an expert has claimed.
They filed into the hall in pairs with Harry following his older brother who led his younger sibling, as well as Princesses Beatrice and Eugenie, Zara Tindall and Peter Phillips, as well as the young Lady Louise Windsor and James, Viscount Severn.
Body language expert Judi James said stone-faced Harry gave "nothing away emotionally", the Sun reports.
She added: "William and Harry were the only siblings to not walk side by side.
"They arrived with William walking first and Harry behind him as the others all paired off.
"Unlike the other grandchildren, William and Harry had the small comfort of the peaks of their hats hiding most of their faces."
She said Harry's mouth was "clamped in a straight line" and he "gave nothing away emotionally".
Judi praised the grandchildren for a "united act of unbelievable self control" as they paid respect to their much-loved grandmother, claiming they all "drew strength" from one another as they surrounded the coffin.
She added that they "adopted their pose it was difficult to even spot them breathing".
And Judi said she had spotted "an air of determination that suggested they were all drawing strength from one another to complete this gesture for their grandmother".
Lady Louise Windsor also caught the attention of many with a subtle and heart-warming nod to her late grandmother and grandfather.
The 18-year-old was spotted wearing a horse pendant to Her Majesty's vigil - a nod to her horse-loving grandparents.