Queen Elizabeth II spent her final moments with Charles and Anne at her bedside, according to reports.
The solemn passing of Britain's longest-reigning monarch came yesterday afternoon, according to 10 Downing Street, with members of the Royal Family rushing up to Balmoral throughout the day after receiving news of her deteriorating condition.
In what would no doubt have been a source of great comfort to her, it is now claimed that the Queen was accompanied by her two eldest children, the now-King Charles III and Anne, Princess Royal at the end of her life.
An insider told MailOnline that both the royal siblings had both been in Scotland this week, and were therefore able to reach the estate in time to say goodbye.
A number of other senior royals were sadly not able to be there with her.
The 96-year-old Queen is understood to have died before Prince William, Prince Andrew, Prince Edward and Sophie Wessex arrived at Balmoral on Thursday afternoon.
Prime Minister Liz Truss was informed about the monarch's death at 4.30pm, according to her official spokesman, while a plane carrying Prince William, the Duke of York, the Earl and Countess of Wessex landed at Aberdeen airport at 3.50pm.
Just after 6.30pm, Buckingham Palace released a statement to confirm the death of Queen Elizabeth II.
Prince Harry arrived at Balmoral later in the evening after flying in to Aberdeen alone, where he appeared sombre in a suit and black tie.
He and Meghan had been due to attend a charity event in London before having to change their plans last minute as a result of the Queen's ill health.
Harry, who was in the UK on the last leg of what was planned to be a brief tour, had not yet seen the Queen or any of the royals during their visit. The California-based couple made an appearance at the One Young World summit in Manchester on Monday.
King Charles will return to London today, where he will meet with officials to discuss plans for the Queen's funeral and is expected to record a speech.
A number of commemorative displays to mark the Queen's passing, including gun salutes, will also take place at various locations in London.