Nathan Patterson is out of Scotland's clash with Ukraine.
The Dark Blues face a huge World Cup semi-final play-off at Hampden tomorrow night.
Victory would set up a 90 minute winner-takes-all clash with Wales for a spot at Qatar 2022 later this year.
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But Steve Clarke will have to do without Everton and ex-Rangers full-back Patterson.
The 20-year-old had ankle surgery at the beginning of April and was given the chance to prove his fitness for the massive showdown.
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But he has failed to gain pass marks and will now miss out in the first match of the hectic international June schedule.
Asked if everyone in the squad was fit, boss Clarke said: "Everyone except Nathan. Nathan has run out of time a little bit for the first game.
"So Nathan won't be involved in the first game but everyone else is good to go."
