Liz Truss has met the King one week after her first awkward encounter with the monarch.
The new PM has been pictured shaking hands with the new head of state in the 1844 room at Buckingham Palace today.
It comes one week after social media users poked fun at Mrs Truss for awkwardly curtsying during her first meeting with King Charles.
His Majesty and the Prime Minister have now met once again ahead of the Queen's funeral tomorrow.
The King greeted Ms Truss at around 12.15pm before holding meetings with the leaders of other Commonwealth countries.
Charles has become the head of state of 14 Commonwealth countries and the UK following his accession.
There is speculation that some Commonwealth countries will seek to remove the monarch as head of state following the Queen's death.
The Queen was respected across Britain's former colonies, however King Charles does not wield the same widespread admiration.
Barbados, which once known as 'little England', finally cut ties with the Queen last year.
Other Caribbean countries, including Jamaica and Antigua and Barbuda, are also preparing to remove the monarch as head of state.
Antigua and Barbuda's Prime minister Gaston Browne said he would push for a referendum on the monarchy following the Queen's death.
Belize has already confirmed a constitutional review.
New Zealand's PM Jacinda Ardern also said she believes her country will become a republic within her lifetime.
However, she added that there were no plans to remove the monarchy "any time soon".
Asked why she has said she believes the move will happen in her lifetime, she told the BBC: "I think even the Queen herself has observed and acknowledged the evolution over time in our relationships.
"My observation is that there will continue to be an evolution in our relationship. I don't believe it will be quick or soon, but over the course of my lifetime."
Ms Ardern also dismissed the "fuss" being made over world leaders having to catch the bus to the Queen's funeral.
Last week the Foreign Office issued guidance to world leaders and royals visiting the capital for the state funeral, urging them to use government-provided shuttle buses, rather than private vehicles.
Some embassies have asked for exceptions but have not been successful, Politico reports.
However, New Zealand's leader Jacinda Arden said her ministers regularly use carpools.
She told the BBC : "I'm kind of interested there's so much fuss about it, I don't think the bus warrants too much fuss. This just makes good sense. We're a very practical people."
No10 has revealed that US President Joe Biden will not be expected to travel on the bus, despite other countries' requests reportedly being rejected.
Biden is to arrive at the state funeral in his famous armoured limousine known as "The Beast".
