King Charles III's first runner had to settle for second behind a horse ridden by the Queen's Gold Cup jockey.
Educator was the 11-10 favourite with the bookmakers to make a winning start for his owner at Salisbury. But he was comfortably overturned by Okeechobee (7-4), the second choice of punters in the four-runner field.
Ryan Moore, who gave the Queen one of her best racing moments when Estimate took the 2013 Gold Cup at Royal Ascot, was in the saddle.