Humza Yousaf dramatically walked off stage at the SNP conference after he was heckled by a protester.
The First Minister was giving his keynote independence speech to supporters at Dundee‘s Caird Hall when an audience member started booing.
The First Minister was interrupted by a woman in the Caird Hall audience, who complained of chronic pain and called for an inquiry into NHS Tayside.
Mr Yousaf said: "Folks, let’s not boo" and exited the stage to try and engage with the protester.
The SNP chief spoke directly to the woman one-on-one in the seating area, pausing proceedings for around five minutes.
The woman then left after being helped out by Mr Yousaf.
Upon returning to the stage, he received a standing ovation from the audience and stressed the importance of listening over shouting down.
In his speech Mr Yousaf said the next Westminster election should be used as a "manifesto for an independent Scotland".
The First Minister told SNP members at the independence convention that the manifesto should read a "vote SNP for Scotland to become independent".
He said: "I am very clear, there is no route to independence except through the lawful and democratic process. Why? Because that is the way that we actually become independent."
He added: "That election gives us the opportunity to break the log jam.
"I believe that in this election, the SNP should offer the people of Scotland a manifesto for an independent Scotland.
"And I am proposing in that manifesto - page one, line one - a simple powerful statement to the people. It should say ‘vote for SNP for an independent Scotland’.
"If the SNP win this election, then the people will have spoken. We will seek negotiations with the UK Government on how we give democratic effect to Scotland becoming an independent nation."