Development in Auchterarder will be stopped after councillors refused plans for a £7 million offslip from the A9 at Shinafoot.
Refusing the application planning convener Ian Massie said PKC wanted to send a "clear message" to developers to fulfil obligations.
Muir Homes and Stewart Milne Group sought permission to create a single rather than dual slip road but said they did so on advice from PKC.