Spider-Man fans don't think two major characters will ever make the jump to the MCU.
Both Harry Osborn and Gwen Stacy had prominent roles in the Tobey Maguire and Andrew Garfield Spider-Men movies, but have yet to appear in the MCU. Harry is the son of Green Goblin, AKA Norman Osborn, and becomes a villain in his own right. Gwen Stacy, meanwhile, is Peter Parker's girlfriend, who dies tragically (not to be confused with Spider-Gwen, a different incarnation of the character via the multiverse and a main character in the animated Spider-Verse movies).