Around 45,000 animals over a five-mile radius have died after a train carrying toxic chemicals crashed.
Environmental officials believe 43,700 aquatic species were killed after the train derailed in Ohio on February 3.
It is more than 10 times the initial estimate, with health boss Mary Mertz claiming 38,222 small fish were killed, as well as 5,500 amphibians and other creatures and type of fish.
